Journal: Biomedicines

Article Title: Mesenchymal Stem Cell–Derived Exosomes Mitigate Cutaneous Radiation Injury Through Coordinated Modulation of DNA Repair, Stress, and Inflammatory Gene Programs

doi: 10.3390/biomedicines14040811

Figure Lengend Snippet: Expression of early damage and stress-response genes ( a ) FDXR , ( b ) GADD45A , ( c ) SESN1 , and ( d ) GDF15 in irradiated HDFs (10 Gy) treated with exosomes (1 × 10 9 particles/mL) derived from BM-MSCs (BM-IR) and UC-MSCs (UC-IR), measured by qPCR. Gene expression was normalized to GAPDH and expressed as fold change relative to the non-irradiated Vehicle control at each timepoint. Data are shown as mean ± SD of three technical replicates from a representative experiment performed using a single HDF donor. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 by ordinary one-way ANOVA followed by Dunnett’s post hoc test, comparing each treatment group to the Vehicle-IR control at the corresponding timepoint.

Article Snippet: Primary human dermal fibroblasts (HDFs) were purchased from PromoCell GmbH (SKU: C-12302; Heidelberg, Germany).

Techniques: Expressing, Irradiation, Derivative Assay, Gene Expression, Control

Journal: Biomedicines

Article Title: Mesenchymal Stem Cell–Derived Exosomes Mitigate Cutaneous Radiation Injury Through Coordinated Modulation of DNA Repair, Stress, and Inflammatory Gene Programs

doi: 10.3390/biomedicines14040811

Figure Lengend Snippet: Expression of DNA repair and antioxidant genes ( a ) DDB2 , ( b ) RNF8 , and ( c ) SOD1 in irradiated HDFs (10 Gy) treated with exosomes (1 × 10 9 particles/mL) derived from BM-MSCs (BM-IR) and UC-MSCs (UC-IR), measured by qPCR. Gene expression was normalized to GAPDH and expressed as fold change relative to the non-irradiated Vehicle control at each timepoint. Data are shown as mean ± SD of three technical replicates from a representative experiment performed using a single HDF donor. * p < 0.05, ** p < 0.01, *** p < 0.001, by ordinary one-way ANOVA followed by Dunnett’s post hoc test comparing each treatment group to the Vehicle-IR control at the corresponding timepoint.

Article Snippet: Primary human dermal fibroblasts (HDFs) were purchased from PromoCell GmbH (SKU: C-12302; Heidelberg, Germany).

Techniques: Expressing, Irradiation, Derivative Assay, Gene Expression, Control

Journal: Biomedicines

Article Title: Mesenchymal Stem Cell–Derived Exosomes Mitigate Cutaneous Radiation Injury Through Coordinated Modulation of DNA Repair, Stress, and Inflammatory Gene Programs

doi: 10.3390/biomedicines14040811

Figure Lengend Snippet: Expression of cell-cycle regulation and proliferation genes ( a ) CDKN1A , ( b ) CDKN2A , ( c ) MKI67 , ( d ) H2AFX , and ( e ) VEGFA in irradiated HDFs (10 Gy) treated with exosomes (1 × 10 9 particles/mL) derived from BM-MSCs (BM-IR) and UC-MSCs (UC-IR), measured by qPCR. Gene expression was normalized to GAPDH and expressed as fold change relative to the non-irradiated Vehicle control at each timepoint. Data are shown as mean ± SD of three technical replicates from a representative experiment performed using a single HDF donor. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 by ordinary one-way ANOVA followed by Dunnett’s post hoc test, comparing each treatment group to the Vehicle-IR control at the corresponding timepoint.

Article Snippet: Primary human dermal fibroblasts (HDFs) were purchased from PromoCell GmbH (SKU: C-12302; Heidelberg, Germany).

Techniques: Expressing, Irradiation, Derivative Assay, Gene Expression, Control

Journal: Biomedicines

Article Title: Mesenchymal Stem Cell–Derived Exosomes Mitigate Cutaneous Radiation Injury Through Coordinated Modulation of DNA Repair, Stress, and Inflammatory Gene Programs

doi: 10.3390/biomedicines14040811

Figure Lengend Snippet: Expression of inflammatory response genes ( a ) IL-6 and ( b ) TNFAIP3 in irradiated HDFs (10 Gy) treated with exosomes (1 × 10 9 particles/mL) derived from BM-MSCs (BM-IR) and UC-MSCs (UC-IR), measured by qPCR. Gene expression was normalized to GAPDH and expressed as fold change relative to the non-irradiated Vehicle control at each timepoint. Data are shown as mean ± SD of three technical replicates from a representative experiment performed using a single HDF donor. * p < 0.05, ** p < 0.01, **** p < 0.0001 by ordinary one-way ANOVA followed by Dunnett’s post hoc test, comparing each treatment group to the Vehicle-IR control at the corresponding timepoint.

Article Snippet: Primary human dermal fibroblasts (HDFs) were purchased from PromoCell GmbH (SKU: C-12302; Heidelberg, Germany).

Techniques: Expressing, Irradiation, Derivative Assay, Gene Expression, Control

Journal: Scientific Reports

Article Title: Chitosan-dextran sulfate nanocapsules for enhanced tigecycline efficacy against non-typhoidal Salmonella enterica

doi: 10.1038/s41598-026-35229-7

Figure Lengend Snippet: TGC and/or unloaded CD NPs ameliorated the S. Typhimurium infected group induced histopathological alterations in mice’s liver tissues. Representative photomicrographs of the H&E-stained hepatic tissue sections showing the control (A), S. Typhimurium infected group (C), TGC (E), unloaded CD NPs (G), CD-TGC groups (I) and their respective higher magnifications (B, D, F, H, and J). A, B: control group displaying normal central vein (CV), hepatic cords (HC) with hepatocytes of eosinophilic granular cytoplasm (EC), rounded central single (SN) or double vesicular nuclei (DN), and kupffer cells (KC). C, D: S. Typhimurium infected group demonstrating multiple areas of variable-sized necrotic areas (NA) of coagulative necrosis (CN), severely dilated and congested sinusoid (SDS) with Kupffer cell hyperplasia (KCH). E, F: TGC group displaying moderately sized necrotic areas (MNA), moderately dilated and congested sinusoids (MDS), moderately hyperplastic Kupffer’s cells (MKC), interstitial mononuclear cell infiltration (MI), fibroblast proliferation (FP), and regenerated hepatocytes of stippling basophilic cytoplasm (BC) and pale nuclei (PN). G, H: unloaded CD NPs group showing a few scattered minute necrotic areas (mNA), intense mononuclear cell infiltration (IMI) around the portal area, and apparently normal hepatocytes (NH). I, J: CD-TGC group showed normal hepatocytes (NH), few dilated blood vessels (DBV), and a few interstitial lymphocytic aggregates (FL). Scale bars = 100 μm in A, C, E, G, I; and = 20 μm in B, D, F, H, and J. K: Bar charts demonstrate the statistical analysis of the comparative quantification of the hepatic injury scores in all studied groups. Bars carrying different superscript letters (a, b, c, d, and e) are significantly different as analyzed by the one-way ANOVA test, followed by the multiple comparisons by Duncan’s Post-hoc test ( p < 0.05). Values are the mean of 6 mice per group ± S.E.M.

Article Snippet: BJ normal human foreskin primary fibroblast cell line (ATCC CRL-2522) was used for studying safety of CD-TGC nanocapsules.

Techniques: Infection, Staining, Control